Towards protecting grapevine using natural bioprotectants
Moore, J P
University of Stellenbosch. Faculty of AgriSciences. Institute for Wine Biotechnology
The South African wine industry needs to develop innovative environmentally friendly technologies to control fungal diseases, such as Botrytis cinerea, in nurseries and vineyards. In this project, biostimulants such as strigolactone treatments were tested as promising natural compounds to enhance the plants immune system in laboratory and greenhouse conditions. Strigolactone preparations (±)-GR24 and Nijmegen-1 were applied as root-soaks to in vitro plantlets of tobacco (Nicotiana tabacum) and grapevine (Vitis vinfera) cv Cabernet Sauvignon; and Cabernet Sauvignon and Sauvignon Blanc nursery vines. Treated plants challenged with Botrytis cinerea were then assessed for lesion size and disease progression.
Strigolactone preparations (±)-GR24 and Nijmegen-1 treated tobacco and grapevine (cv Cabernet Sauvignon) in vitro plantlets and glasshouse plantlets showed decreased susceptibility to Botrytis cinerea. Treatment of grafted nursery vines (cv Cabernet Sauvignon and Sauvignon Blanc) showed no impact on disease susceptibility to Botrytis cinerea in glasshouse conditions. Strigolactone preparations (±)-GR24 and Nijmegen-1 have potential as bioprotectants by influencing plant-fungus interactions in grapevine plantlets. Strigolactone preparations could form part of a bioprotectant treatment programme in combination with other plant hormones used on grapes. Further research is necessary to assess the molecular mechanism-of-action in planta. Strigolactone preparations (±)-GR24 and Nijmegen-1 are biostimulants and plant hormones that could help prevent vine losses in grapevine nurseries. These preparations should be tested in vine nursery trials to confirm this.
Dominic Vogel. 2019. (MSc in Wine Biotechnology) Evaluating the role of natural plant derived compounds in modifying disease defence mechanisms in Nicotiana tabacum and Vitis vinifera plantlets (Supervisor with Dr Paul Hills) Stellenbosch University, MSc thesis (online open access, embargoed)
Florent Weiller. 2020. (PhD in Wine Biotechnology) Cell wall profiling of tobacco and grapevine in the context of Botrytis cinerea infection. Stellenbosch University, PhD thesis in preparation
Dominic Vogel, Paul N. Hills, John P. Moore. 2017. Evaluating the role of natural plant derived compounds in modifying disease defence mechanisms in Nicotiana tabacum and Vitis vinifera plantlets in South African Journal of Botany, South African Association of Botanists Conference, 8-11th January, Cape Town, South Africa