The development of an ELISA diagnostic kit for grapevine fanleaf nepovirus


Project Number
GENUS 05-01

Project title
The development of an ELISA diagnostic kit for grapevine fanleaf nepovirus

Project leader
Burger, J T

Institution
University of Stellenbosch. Department of Genetics

Team members
Bellstedt, D
Kerr, M S
De Villiers, C A

Project description
Grapevine fanleaf disease is caused by a member of the nepovirus family and transmitted by the soil nematode, Xiphinema index. The disease is most prevalent in the Breede River Valley region, an area with high Xiphinema infestations. The Vine Improvement Association (VIA) directives for certification of grapevine propagation material (nuclear and mother block material) prescribe that vines need to be tested by ELISA for the presence of Grapevine fanleaf nepovirus (GFLV). Diagnostic kits for this purpose, based on Enzyme-Linked Immunosorbent Assay (ELISA) were previously provided locally, but this source of kits will become unavailable at the end of 2006. We propose to prepare GFLV-specific antibodies from recombinant GFLV coat protein (CP), expressed in Escherichia coli, for use in a locally developed ELISA diagnostic ki

FinalReport.pdf

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