Project Number
IWBT 04-02

Project title
The construction of genomic and cDNA libraries of grapevine cultivars

Project leader
Vivier, M A

Institution
University of Stellenbosch. Faculty of AgriSciences. Institute for Wine Biotechnology

Team members
Young, P

Project description
Genomic and cDNA libraries can be viewed as genetic references for the specific grape cultivar being used as source material. Many biotechnological projects rely on the presence of representative libraries and whereas a genomic library is representative of the total genomic composition of a specific cultivar, a cDNA library represents only genes that are expressed in the tissues that were used as source material. The latter is useful when the purpose is to isolate genes that are only differentially expressed. Both a cDNA and a genomic library have been constructed from Pinotage in this project and are currently screened for useful genes and promoters.

Genomic and cDNA libraries are resources in plant biotechnology to isolate genes and promoters from and to analyse fundamental aspects of genome organisation of a plant species. The objectives of this study was to construct a genomic DNA library from Pinotage as well as to construct a cDNA library from leaves of Pinotage.

Poster(s)
Champanis, R, Vivier, M A, Botha, F C and Pretorius I S. 1999. Manipulation of grapevine sugar metabolism. Poster. 23rd National Congress of the South African Society for Enology and Viticulture, Somerset West, South Africa, 12-13 November

Champanis R, Vivier, M A, Botha, F C and Pretorius, I S. 2000. Transformation of grapevine with a yeast-derived invertase (SUC2) targeted to different cellular locations. Poster. BioY2K: Combined Millennium Meeting, Grahamstown.

Presentation(s)
Champanis, R, Vivier, M A, Botha, F C and Pretorius, I S. 1998. Sweetening up to Vitis. Presentation at the Meeting of the Experimental Biology Group of South Africa, Cape Town.

Young, P R, Vivier, M A and Pretorius, I S. 1998. Isolation and characterisation of a ß-carotene hydroxylase from Vitis vinifera. Paper presented at the 2nd Congress of the Federation of African Societies of Biochemistry and Molecular Biology, Potchefstroom.

Young, P R, Vivier, M A and Pretorius, I S. 1999. Sequence analysis of a ß-carotene hydroxylase from Vitis vinifera. Paper presented at the 23rd National Congress of the South African Society for Enology and Viticulture, Somerset West, South Africa, 12-13 November

Young, P R, Vivier, M A and Pretorius, I S. 2000. The structural analysis of a ß-carotene hydroxylase-encoding gene from Vitis vinifera. Paper presented at the BioY2K Combined Millennium Meeting, Grahamstown.

Young, P R, Pretorius, I S and Vivier, M A. 2000. The structural analysis of a beta-carotene hydroxylase-encoding gene from Vitis Vinifera. Paper presented at the BioY2K, Combined Millennium Meeting, Grahamstown.

Young, P R, Pretorius, I S and Vivier, M A. 2001. Isolation and characterisation of carotenoid biosynthesis genes from Vitis vinifera. Paper presented at the 26th World Congress and the 81st General Assembly of the International Office of Vine and Wine, Australia.

Young, P R, Taylor, K L, Chen, S W, Pretorius, I S and Vivier, M A. 2001. Elucidation and manipulation of carotenoid biosynthesis in Vitis vinifera. Paper presented at the 26th World Congress of the Vine and Wine and the General Assembly of the OIV and Wine OIV, Adelaide, Australia, 11-17 October

FinalReport.pdf