The Construction of an Infectious Clone of Grapevine Virus A


Project Number
GENUS 01-05

Project title
The construction of an infectious clone of grapevine virus A

Project leader
Burger, J T

Institution
University of Stellenbosch. Department of Genetics

Project description
Shiraz decline is an emerging disease of grapevine in the Western Cape with very distinctive symptoms, and a crippling effect in vineyards. Infected vines deteriorate relatively fast and normally die within five years. The etiology of the disease is largely unknown, but is believed to be of multiple viral origin with Grapevine virus A (GVA) consistently associated with the disease (pers. comm. D. Goszczynski). The aim of the project is to investigate GVA’s role in this interaction by creating a tool to study pathogen-host interactions on a molecular level. We aim to construct a full-length infectious cDNA clone of the virus and to ultimately develop a transient expression vector for grapevine to assist in elucidating the etiology of this disease, and possibly obtaining resistance to Shiraz disease.

Our objective for the first year is to construct an infectious clone of a local isolate of GVA. We plan to isolate and clone contiguous fragments of the viral genome by RT-PCR and to assemble these into a complete and infectious clone.

Poster(s)
Van Eeden, C and Burger, J T. 2002. A study towards the introduction of multiple virus resistance in grapevine through PTGS. Poster presented at the 40th Congress of the Southern African Society for Plant Pathology. 20-23 January, Dikhololo, South Africa.

Van Eeden, C and Burger, J T. 2002. The introduction of multiple virus resistance in grapevines through virus-induced gene silencing. Poster presented at the Cape Biotechnology Forum. September, Somerset West, South Africa.

Van Eeden, C, Freeborough, M-J and Burger, J T. 2004. The introduction of multiple virus resistance in grapevines using PTGS. Poster presented at the 18th Congress of the South African Genetics Society. 4-7 April, Stellenbosch, South Africa

Burger, J T and Engelbrecht, M. 2004. The construction of a tandem silencing vector for grapevine. Poster presented at the 18th Congress of the South African Genetics Society, 4-7 April, Stellenbosch, South Africa.

Du Preez, J, Freeborough, M-J and Burger, J T. 2005. Exploiting the GVA genome as a somatic expression vector in grapevine. Poster presented at the International Grape Genomics Symposium. St Louis, MO, United States.

Presentation(s)
Du Preez, J and Burger, J T. 2002. The construction of an infectious clone of grapevine virus A. Paper presented at the 26th National Congress of the South African Society for Enology and Viticulture. 13-15 November, Somerset West, South Africa

Burger, J T, Engelbrecht, M and Van Eeden, C. 2003. The construction of gene silencing vectors for the introduction of multiple virus resistance in grapevines. Paper presented at the 14th Congress of the International Council for the Study of Virus-like Diseases of Grapevine. 12-17 September, Locorotondo, Bari, Italy.

Du Preez, J, Freeborough, M-J and Burger, J T. 2005. The construction of an RNA virus-based expression vector for grapevines. Paper presented at the 3rd International Congress of the South African Society for Enology and Viticulture. 28-30 July, Cape Town, South Africa.

Du Preez, J, Freeborough, M-J and Burger, J T. 2006. The construction of an RNA virus-based expression vector for grapevine. Paper presented at the 14th Plant and Animal Genome Conference. San Diego, California, United States.

FinalReport.pdf

Generic selectors
Exact matches only
Search in title
Search in content
Post Type Selectors