Survey of white and red cultivar vineyards affected by grapevine leafroll for generic variation in grapevine leafroll-associated virus 3


Project Number
GENUS 11-01

Project title
Survey of white and red cultivar vineyards affected by grapevine leafroll for generic variation in grapevine leafroll-associated virus 3.

Project leader
Burger, J T

Institution
University of Stellenbosch. Faculty of AgriSciences. Department of Genetics

Team members
Bester, R
Maree, H J
Jooste, A E C
Burger, J T
Stephan, D

Project description
It is important to have reliable detection techniques that would detect all GLRaV-3 genetic variants present in our vineyards and to establish reference plants for each variant.

Most research on grapevine leafroll disease has been conducted on red wine cultivars, while genetic variation of GLRaV-3 in white cultivars remains largely unknown. This project will address this lack of information by determining the genetic diversity of GLRaV-3 in a widespread survey on white and red cultivars. The prevalence of the newly identified strain of GLRaV-3 (SANZ) will be determined in the same survey. The ability of existing industry standard diagnostic ELISA tests to detect all genetic variants will also be evaluated.

Presentation(s)
Bester, R, Jooste, A E C, Maree, H J and Burger, J T. 2012. Real-time RT-PCR high-resolution melting curve analysis and multiplex RT-PCR to detect and differentiate grapevine leafroll-associated associated virus 3 variant groups I, II, III and VI. Paper presented at the 17th International Council for the Study of Virus and Virus-like Diseases of the Grapevines. 7-14 October, Davis, California, United States.

Jooste, A E C, Bester, R, Maree, H J, De Koker, W and Burger, J T. 2012. A survey of red and white cultivars to test an improved detection technique for GLRaV-3 variants identified in South African vineyards. Paper presented at the 17th Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevines. 7-14 October, Davis California, United States.

Jooste, E and Bester, R. 2012. A survey in white and red cultivars to determine the spread of GLRaV variants in vineyards. Paper presented at the 12th Grapevine Virus Workshop. ARC Nietvoorbij-Infruitec, Stellenbosch, South Africa.

Bester, R, Jooste, A E C, Maree, H J and Burger, J T. 2012. Sequencing and detection of a new strain of grapevine leafroll-associated virus 3 in South Africa. Paper presented at the 34th National Congress of the South African Society for Enology and Viticulture. 14-16 November, Somerset West, South Africa.

Article
Bester, R, Jooste, A E C, Maree, H J, Burger, J T. 2012. Real-time RT-PCR high-resolution melting curve analysis and multiplex RT-PCR to detect and differentiate grapevine leafroll-associated virus 3 variant groups I, II, III and IV, Virology Journal, v. 9 (p. 219)
http://dx.doi.org/10.1186/1743-422X-9-219

Jooste, A E C, Molenaar, N, Maree, H J, Bester, R, Morey, L, De Koker, W C, Burger, J T. 2015. Identification and distribution of multiple virus infections in grapevine leafroll diseased vineyards, European Journal of Plant Pathology, Mnth Feb
http://dx.doi.org/10.2007/s10658-015-0620-0

Paper
Bester, R, Jooste, A E C, Maree, H J, Burger, J T. 2012. Real-time RT-PCR high-resolution melting curve analysis and multiplex RT-PCR to detect and differentiate grapevine leafroll-associated associated virus 3 variant groups I, II, III and VI, IN: Furguson, B (ed). Proceedings of the 17th Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine. 7-14 October 2012, Davis, California, United States. (p. 140-141) Foundation Plant Services University of California, Davis, California.

Jooste, A E C, Bester, R, Maree, H J, De Koker, W, Burger, J T. 2012. A survey of red and white cultivars to test an improved detection technique for GLRaV-3 variants identified in South African vineyards, IN: Furguson, B (ed). Proceedings of the 17th Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine. (p. 122-123) 7-14 October 2012, Foundation Plant Services University of California, Davis, California.
http://dx.doi.org/10.13140/2.1.5115.4885

Thesis
Bester, R. 2012. Sequencing and detection of a new strain of grapevine leafroll associated virus III in South Africa. MSc (Genetics) University of Stellenbosch, Stellenbosch.

FinalReport.pdf

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