US GMB 02
Soil micro-organisms as bio-indicators of copper induced stress
University of Stellenbosch. Department of Microbiology
Copper, which is administered to vines as a component of fungicides, ends up in the soil of vineyards and nurseries. The aim of the project is to determine the threshold concentration of copper in soils, which if exceeded, would result in a detrimental effect on soil microorganisms. Consequently, the impact of a series of copper concentrations is determined on microbial communities in different soils. The communities studied include total numbers of cultivable bacteria and fungi, as well as selected bacterial and fungal groups. In addition, the impact of copper on the metabolic potential of the total microbial community, protozoan numbers and the mycorrhiza of young vines is being assessed. Consequently, the foundations are laid for the development of methods using biological indicators to detect copper induced stress. It is planned that this detection system will eventually enable the farmer to take precautionary measures to prevent deterioration of productive soil.
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Wolfaardt, G M and Botha, A. 2000. Microbial responses to copper on soil. Paper presented at the 25th Meeting of the Canadian Society of Microbiologists. 11-14 June, University of Manitoba, Winnipeg, Canada.
Cornelissen, C, Botha, A, Wolfaardt, G M, Conradie, W J, Louw, P J E and Botha, G. 2000. The impact of copper on microbial populations present in fynbos soil. Presentation to the Fynbos Forum. 7-9 June, Ganzekraal, South Africa.
Botha, A, Wolfaardt, G M, Conradie, W J, Van Huyssteen, and Olivier, M P. 2001. The impact of copper on soil microbes. Paper presented at the 25th National Congress of the South African Society for Enology and Viticulture. 15-16 November, Somerset West, South Africa.
Cornelissen, S, Botha, A, Wolfaardt, G M and Conradie, W J. 2002. The impact of copper on fungal populations present in soil of the Western Cape. Paper presented at the 12th Congress of the South African Society for Microbiology. 2-5 April, Bloemfontein, South Africa.
Du Plessis, K R, Botha, A and Wolfaardt, G M. 2002. The impact of copper oxychloride on selected eukaryotic and prokaryotic microbial populations in soil. Paper presented at the 12th Congress of the South African Society for Microbiology. 2-5 April, Bloemfontein, South Africa.
Cornelissen, S, Botha, A, Conradie, W J, Wolfaardt, G M. 2003. Shifts in community composition provide a mechanism for maintenance of soil yeast activity in the presence of elevated copper levels, Canadian Journal of Microbiology, v. 49 (7) (p. 425-432)
Du Plessis, K R, Botha, A, Joubert, L, Bester, R, Conradie, W J, Wolfaardt, G M. 2005. Response of the microbial community to copper oxychloride in acidic sandy loam soil, Journal of Applied Microbiology, v. 98 (4) (p. 901-909)