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Molecular and Metabolite Profiling of Grapevines in Vineyard Settings

by | Oct 25, 2020 | Uncategorized

Project Number
IWBT 2012-01

Project title
Molecular and metabolite profiling of grapevines in vineyard settings

Project leader
Vivier, M A

Institution
University of Stellenbosch. Faculty of AgriSciences. Institute for Wine Biotechnology

Team members
Vivier, M A
Young, P R
Jacobson, D
Hunter, K
Deloire, A
Strever, A E
Da Silva Ferreira, A
Eyeghe-Bickong, H
Gouws, L

Project description
One of the problems facing most viticultural studies is that it is difficult to explain the link between an action or impact (biotic or abiotic) in the vineyard and the associated impact on the grapes and wine.

It is now possible to implement molecular biology tools in the study of grapevines in vineyard settings. The basic information generated regarding the growth and development of the grapevine with these tools is extremely valuable to explain why a specific response is obtained, specifically when the study is conducted within the framework of a broader viticultural study.

The objectives of the study are to perform molecular and metabolite profiling of grapevines in vineyard settings to understand how environmental factors impact vine biology and influence quality traits. The proposed work is planned in collaboration with and in support of existing viticultural studies and will focus on the effect of light and temperature on grape development or composition.

Understanding how a grapevine adapts on a molecular level to its physical environment (due to viticultural management practices or abiotic, biotic stresses) is a prerequisite if predictive measures of plant status and/or berry quality are to become part of the vineyard management system. The ability to analyse the grapevine molecular ‘phenotype’ in a vineyard setting will assist researchers in quantifying the impact of viticultural practices, leading to informed decisions regarding these practices and their improvement. This will significantly advance our understanding of the processes involved, predisposing factors as well as possible ways to protect grapes against adverse climatic conditions. Ultimately, this knowledge could lead to predictive markers; instruments to monitor ripening and stress impact factors during this period, and improved management practices.

Poster(s)
Musingarawbi, D, Eyeghe-Bickong, H A, Young, P R, Nieuwoudt, H H and Vivier, M A. 2014 Rapid techniques for assessment of grapes and wine. Poster presented at 5th International Symposium on Macromolecules and Secondary Metabolites of Grapevine and Wines: MacroWine 2014. 7-10 September 2014, Stellenbosch, South Africa.

Du Plessis, K, Young, P R and Vivier, M A. 2014. A highly characterised model vineyard approach towards effective implementation of field-omics in grapevine studies. Poster presented at 5th International Symposium on Macromolecules and Secondary Metabolites of Grapevine and Wines: MacroWine 2014. 7-10 September 2014, Stellenbosch, South Africa.

Presentation(s)
Young, P E, Alexandersson, E, Jacobson, D, Lashbrooke, J, Coetzee, A J, Deloire, A J and Vivier, M A. 2012. The molecular and metabolite profiling of grapevine berries in a model vineyard where the microclimate of the developing bunches has been altered. Paper presented at 38th Conference of the South African Association of Botanists. 15-18 January, University of Pretoria, Pretoria, South Africa.

Vivier, M A, Young, P R, Alexandersson, E, Jacobson, D, Lashbrooke, J, Coetzee and Deloire, A J. 2012. The molecular response of grapevine berries to an altered microclimate: The effect of leaf removal and sunlight exposure in the bunch zone on the carotenoid biosynthetic pathway. Paper presented at the 23rd Congress of the South African Society of Biochemistry and Molecular Biology and Federation of African Societies of Biochemistry and Molecular Biology. 29 January – 1 February 2012. Drakensberg, South Africa.

Young, P R, Alexandersson, E, Jacobson, D, Lashbrooke, J, Eyeghe-Bickong, H, Coetzee, Z A, Coetzee, C, Kritzinger, E, Du Toit, W, Deloire, A J and Vivier, M A. 2012. Profiling of carotenoid metabolism and quality parameters in grapes and wines from a model vineyard where the microclimate of the developing bunches has been altered. Paper presented at the Macrovision of Macrowine 2012 Conference: Viticulture, winemaking and markets. 8-21 June, Bordeaux, France.

Vivier, M A. 2013. Trends in grapevine biology. Paper presented at the 35th South African Society for Enology and Viticulture Conference. 13-15 November, Somerset West, South Africa.

Young, P R and Maree, M A. 2013. Grape flavours uncorked. Paper presented at the 35th South African Society for Enology and Viticulture Conference. 13-15 November, Somerset West, South Africa.

Vivier, M A. 2013. From vineyard to grapes – understanding the impact of viticultural treatments. Paper presented at the 35th South African Society for Enology and Viticulture Conference. 13-15 November, Somerset West, South Africa.

Young, P R, Lashbrooke, J G, Dockrall, S J and Vivier, M A. 2013. Functional analysis of carotenoid cleavage dioxygenases in grapevine. Paper presented at the Carotenoids Gordon Research Conference. Ventura, California, United States.

Young, P R, Lashbrooke, J G, Dockrall, S J, Alexandersson, E, Jacobson, D and Vivier, M A. 2013. Grapevine carotenoids and carotenoid cleavage dioxygenases: Expression profiling and functional analysis. Paper presented at the 9th International Symposium on Grapevine Physiology and Biotechnology. 21-26 April, La Serena, Chile.

Vivier, M A. 2014. Systems biology of grapevines in field settings using model vineyards. Paper presented at the Plant Gene Discovery and Omics Technology Conference. 17-18 February, Vienna, Austria.

Vivier, M A, Young, P R, Du Plessis, K, Eyeghe-Bickong, H and Joubert, C. 2014. The molecular and metabolite profiling of grapevine berries in a model vineyard where the microclimate of the developing bunches has been altered. Paper presented at the 11th International Conference on Grapevine Breeding and Genetics. 28 July – 2 August, Beijing, China.

Eyeghe-Bickong, H, Young, P R and Vivier, M A. 2014. A combination of chromatographic methods to accurately profile and quantify major metabolites in grapevine (Vitis vinifera cv.) berries from characterised vineyards using small sample sizes. Paper presented at 5th International Symposium on Macromolecules and Secondary Metabolites of Grapevine and Wines: MacroWine 2014. 7-10 September 2014, Stellenbosch, South Africa.

Young, P R, Eyeghe-Bickong, H, Jacobson, D, Alexandersson, E, Coetzee, Z A, Deloire, A and Vivier, M A. 2014. Grapevine plasticity in response to light: Sauvignon blanc modulates its berry metabolome in response to an altered microclimate. Paper presented at 5th International Symposium on Macromolecules and Secondary Metabolites of Grapevine and Wines: MacroWine 2014. 7-10 September 2014, Stellenbosch, South Africa.

Article
Eyeghe-Bickong, H A, Alexandersson, E O, Gouws, L M, Young, P R, Vivier, M A. 2012. Optimisation of an HPLC method for the simultaneous quantification of the major sugars and organic acids in grapevine berries, Journal of Chromatography B, Mnth Feb v. 885-886 (p. 43-49)
http://dx.doi.org/10.1016/j.jchromb.2011.12.011

Young, P R, Lashbrooke, E, Alexandersson, D, Jacobson, C, Moser, R, Velasco, R, Vivier, M A. 2012. The genes and enzymes of the carotenoid metabolic pathway in Vitis vinifera L, BMC Genomics, v. 13 (p. 243)
http://doi.org/10.1186/1471-2164-13-243

Alexandersson, E O, Jacobson, D, Vivier, M A, Weckwerth, W, Andreasson, E. 2014. Field-onomics – understanding large scale molecular data from field crops, Frontiers in Science, v. 5 (p. 286)
http://doi.org/10.3389/fpls.2014.00286

Musingarabwi, D M, Nieuwoudt, H H, Young, P R, Eyeghe-Bickong, H A, Vivier, M A. 2016. A rapid qualitative and quantitative evaluation of grape berries at various stages of development using Fourier-transform infrared spectroscopy and multivariate data analysis, Food Chemistry, Mnth Jan v. 190 (1) (p. 253-262)
http://doi.org/10.1016/j.foodchem.2015.05.080

Young, P, Eyeghe-Bickong, H A, Du Plessis, K, Alexandersson, E, Jacobson, D A, Coetzee, Z A, Deloire, A, Vivier, M A. 2016. Grapevine plasticity in response to an altered microclimate: Sauvignon Blanc modulates specific metabolites in response to increased berry exposure, Plant Physiology, v. 170 (3) (p. 1235-54)

Chapter
Guedes de Pinho, P, Vivier, M A, Young, P R, Oliveira, C M, Martins, R C, Silva Ferreira, A C. Winterhalter, P Ebeler, S (ed). 2013. Monitoring carotenoids and derivative compounds in grapes and port wines: Impact on quality, IN: Carotenoid Cleavage Products. (p. 139-154) American Chemical Society,

FinalReport.pdf

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