Metabolic Engineering of Grapevine Towards Enhanced Abiotic Stress Resistance and Improved Quality Parameters


Project Number
IWBT-P 08-13

Project title
Metabolic engineering of grapevine towards enhanced abiotic stress resistance and improved quality parameters

Project leader
Young, P R

Institution
University of Stellenbosch. Faculty of AgriSciences. Institute for Wine Biotechnology

Team members
Lashbrooke, J
Tredoux, M
Rao, S
Stander, C
Lerm, T

Project description
The specific aims of this project relates to the understanding and manipulation of core metabolic pathways in grapevine to not only provide insight into how grapevine deals with abiotic stresses, but also to provide alternative strategies and/or novel plant material more resistant to these stresses. Moreover, the project also aims to contribute towards a fully characterised vineyard where the cause(s) and effects of environmental stresses can be separated and studied. The metabolic pathways that will be studied in this project (carotenoid pathway and ascorbic acid/tartrate pathway) are also intricately involved in quality aspects and some of the aims and outcomes relates to functional analysis of impact genes and their encoded products to improve flavour and aroma production in grapevine tissues and berries.

Poster(s)
Young, P R, Alexandersson, E, Jacobson, D, Lashbrooke, J, Coetzee, Z A, Deloire, A J and Vivier, M A. 2011. The molecular response of grapevine berries to an altered microclimate: the effect of leaf removal / sunlight exposure in the bunch zone on the carotenoid biosynthetic pathway. Poster presented at the Systems Biology to Sustainable Agriculture Conference. 17-18 November, Limassol, Cyprus.

Vivier, M A, Young, P R, Alexandersson, E, Jacobson, D, Lashbrooke, J, Coetzee, Z A and Deloire, A J. 2012. The molecular response of grapevine berries to an altered microclimate: the effect of leaf removal in the bunch zone on the carotenoid biosynthetic pathway. Poster presented at the SASBMB-FASBMB Congress. 29 January – 1 February, Drakensberg, South Africa.

Presentation(s)
Becker, J, Young, P R and Vivier, M A. 2007. The mevalonate and terpenoid pathways are induced in response to Botrytis cinerea in both tobacco and grapevine. Paper presented at the 14th International Botrytis Symposium. 21-26 October, Cape Town, South Africa.

Becker, J, Young, P R and Vivier, M A. 2008. The mevalonate and terpenoid pathways are induced in response to Botrytis cinerea in both tobacco and grapevine. Paper presented at the Bio08 Congress. 21-25 January, Grahamstown, South Africa.

Young, P R, Taylor, K and Vivier, M A. 2008. The carotenoid biosynthetic pathway of Vitis vinifera. Paper presented at the Bio08 Congress. 21-25 January, Grahamstown, South Africa.

Lashbrooke, J G, Young, P R Tredoux, A G J, Vasanth, K and Vivier, M A. 2008. Functional analysis of grapevine carotenoid cleavage dioxygenase (VvCCD1) using over-expression and silencing strategies. Paper presented at the 8th International Symposium on Grapevine Physiology and Biotechnology. 18-23 November, Adelaide, Australia

Young, P R, Lashbrooke, J G and Vivier. 2008. Biotechnological potential of carotenoid biosynthetic genes isolated from Vitis vinifera L. Presentation to the Cape Biotechnology Forum. 30 November – 2 December, Somerset West, South Africa

Young, P R, Moore, J P and Vivier, M A. 2010. The role of carotenoid genes in light and drought stress towards a system approach to abiotic stress tolerance in grapevine. Paper presented at the 3th Meeting of FA0605: Plant abiotic stress: from signalling to crop improvement. 26-27 May, Valencia, Spain.

Vivier, M A. 2010. Genetics and genomic approaches to improve grape quality for winemaking. Paper presented at the International Intervitis Interfructa Congress. 24-28 March, Stuttgart, Germany.

Vivier, M A and Young, P R. 2011. GMO grapevines: excellent study models and promising (future) products. Paper presented at the 2nd International Society for Horticulture Science and Genetically Modified Organisms in Horticulture. 11-15 November, Nelspruit, South Africa.

Young, P R, Alexandersson, E, Jacobson, D, Lashbrooke, J, Coetzee, Z A, Deloire, A J and Vivier, M A. 2012. The molecular and metabolite profiling of grapevine berries in a model vineyard where the microclimate of the developing bunches has been altered. Paper presented at the 38th Conference of the South African Association of Botanists. 15-18 January, University of Pretoria, Pretoria, South Africa

Article
Lashbrooke, J G, Young, P R, Strever, A E, Stander, C, Vivier, M A. 2010. The development of a method for the extraction of carotenoids and chlorophylls from grapevine leaves and berries for HPLC profiling, Australian Journal of Grape and Wine Research, Mnth Jun v. 16 (2) (p. 349-360)

Ramaschandra, S G, Stander, C, Jacobson, D, Ndimba, B K, Vivier, M A. 2011. Proteomic analysis of grape berry cell cultures reveals that developmentally regulated ripening related processes can be studied using cultured cells, PloS One, v. 6 (2) (p. e14708)

Eyeghe-Bikong, H A, Alexandersson, E O, Gouws, L M, Young, P R, Vivier, M A. 2012. Optimisation of an HPLC method for the simultaneous quantification of the major sugars and organic acids in grapevine berries, BMC Genomics, Mnth 13 (p. 243)

Young, P R, Lashbrooke, J G, Alexandersson, E O, Jacobson, D, Moser, C, Velasco, R, Vivier, M A. 2012. The genes and enzymes of the carotenoid metabolic pathway in Vitis vinifera L, BMC Genomics, v. 13 (p. 243)

Chapter
Young, P R, Vivier, M A. Reynolds, A G (ed). 2010. Genetic and genomic approaches to improve grape quality in winemaking, IN: Managing wine quality: Viticulture and wine quality. Vol 1. Woodhead Publishing Limited,

Dissertation
Taylor, K L. 2007. Isolation and character of carotenoid biosynthetic genes from Vitis vinifera. PhD. University of Stellenbosch, Stellenbosch, South Africa.

Paper
Bauer, F F, Naes, T, Esbensen, K, Young, P R, Du Toit, M, Vivier, M A. 2008. Functional wine-omics, IN: Williams, P J (ed). 2007. Proceedings of the 13th Australian Wine Industry Technical Conference. 28 July – 2 August 2007, Adelaide, Australia. (p. 178-183)

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