Isolation and characterisation of fruit specific promotors and identification of alternative systems for transgenic plants


Project Number
IPB 04-04

Project title
Isolation and characterisation of fruit specific promotors and identification of alternative systems for transgenic plants

Project leader
Groenewald, J H

Institution
University of Stellenbosch. Institute for Plant Biotechnology

Team members
Groenewald, S
Venter, M
Swiegelaar, J P

Project description
Successful genetic manipulation of grapevine requires useful genes to confer specific traits and suitable genetic elements to regulate transgene expression.

The goal of the project is three-fold namely
1. the characterisation of genes involved in grapevine ripening
2. the isolation and characterisation of fruit specific promoter elements for application in grapevine genetic transformation
3. the identification of an alternative (non-antibiotic) selection system for transformed grapevine to address environmental and health concerns about the antibiotic selection systems currently in use.

Presentation(s)
Burger, A L and Botha, F C. 2002. Characterisation of a fruit specific, ripening related cDNA from Vitis vinifera cv. Merlot. Presentation at the 28th Conference of the South African Association of Botanists. Rhodes University, Grahamstown, South Africa.

Zwiegelaar, J P and Botha, F C. 2002. Using in situ hybridisation to characterise gene expression in grape berries. Paper presented at the 28th Conference of the South African Association of Botanists. Rhodes University, Grahamstown, South Africa.

Burger, A L, Watts, L and Botha, F C. 2004. Isolation, characterisation and functional analysis of a ripening related promoter element from Vitis vinifera cv. Merlot. Paper presented at the 7th International Symposium on Grapevine Physiology and Biotechnology. 21-25 June, UC Davis, California, United States.

Groenewald, H, Botha, F, Botha, H, Groenewald, S, Hilten, F, Venter, M, Vermaak, C and Zwiegelaar, C. 2004. Wine, biotechnology and the IPB. Presentation at the Winetech Biotechnology Open Day. 4 June, Stellenbosch, South Africa.

Venter, M, Burger, A L, Zwiegelaar, C, Potgieter, B, Botha, F C and Groenewald, J H. Integration of genetic data enhances understanding of gene expression. Joint 18th Congress of the South African Genetic Society and 13th Congress of the South African Microbiology Society. 4-7 April, Stellenbosch, South Africa.

Burger, A L and Botha, F C, Burger, A L and Botha, F C. 2005. Isolation, characterisation and functional analysis of a ripening related promoter from Vitis Vinifera cv. Merlot. Paper presented at the 31st Congress of the South African Association of Botanists. January, University of the Orange Free State, Bloemfontein, South Africa.

Burger, A L, Venter, M and Botha, F C. 2006. An integrative in silico strategy for further insight into ripening related gene regulation in grapevine. Paper presented at the 32nd Congress of the South African Association of Botanists. January, Nelson Mandela Metropolitan University, South Africa.

Article
Burger, A L, Botha, F C. 2004. Cloning of a specific ripening-related gene from the multiple of ripening related genes identified from a single band, excised from a cDNA-AFLP gel, Plant Molecular Biology Reporter, v. 22 (3) (p. 225-236)

Burger, A L, Zwiegelaar, J P, Botha, F C. 2004. Characterisation of the gene encoding the Merlot ripening-induced protein 1: evidence that this puntative protein is a distinct member of the plant proline rich protein family, Plant Science, v. 167 (5) (p. 1075-1089)

Burger, A L, Botha, F C. 2004. Ripening related gene expression during fruit ripening in Vitis vinifera L. cv Cabernet Sauvignon and Clairette blanc, Vitis, v. 43 (2) (p. 59-64)

Venter, M, Botha, F C. 2004. Promoter analysis and transcription profiling: Integration of genetic data enhances understanding of gene expression, Physiologia Plantarium, v. 120 (1) (p. 74-83)

Burger, A L. 2005. Proving more versatile than just ‘strawberries and cream’? WineLand, Mnth May (p. 82-84)

Burger, A L. 2005. Providing more versatile than just ‘strawberries and cream’. The use of strawberries in the genetic manipulation of grapevine fruit metabolism, Wynboer Technical Yearbook, (p. 82-84)

Burger, A L, Watts, L, Botha, F C. 2006. Grapevine promoter directs gene expression in the nectarines of transgenic tobacco, Physiologia Plantarium, v. 126 (3) (p. 418-434)

Venter, M, Groenewald, J-H, Botha, F C. 2006. Sequence analysis and transcriptional profiling of two vacuolar H-pyrophosphatase isoforms in Vitis vinifera, Journal of Plant Research, v. 119 (5) (p. 469-478)

Dissertation
2004. The isolation of grapevine promotors with special emphasis on the VPPase promoter. PhD. University of Stellenbosch, Stellenbosch, South Africa.

Burger, A L. 2004. Isolation and characterization of a developmentally regulated gene from Vitis vinefera berries. PhD. University of Stellenbosch, Stellenbosch, South Africa.

Thesis
2002. Differential gene expression during ripening of Chardonnay berries: Isolation of specific sequences through subtraction. MSc (Wine Biotechnology) University of Stellenbosch, Stellenbosch.

Zwiegelaar, J P. 2002. Tissue specific gene expression in grape berries. MSc (Wine Biotechnology) University of Stellenbosch, Stellenbosch.

FinalReport.pdf

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