Grapevine Biotechnology: The Use of Various Antifungal Genes to Regulate the Plant's Natural Disease Response


Project Number
IWBT 05-08B

Project title
Grapevine biotechnology: The use of various antifungal genes to regulate the plant’s natural disease response

Project leader
Vivier, M A

Institution
University of Stellenbosch. Faculty of AgriSciences. Institute for Wine Biotechnology

Project description
This project has as aim to deliver transgenic grapes with improved resistance against fungal pathogens. Towards this end, several aspects are addressed, including optimisation of transformation and regeneration schemes of grapevine as well as the development and testing of antifungal strategies based on knowledge gained from existing transgenic model plant lines with enhanced fungal resistance. A range of technologies is employed to address these aspects.

Seven genetic constructs have been introduced into grapevine cultivars and several putatively transformed transgenic lines have been regenerated with the prospect to obtain many more from these viable and prolific transformation plates.

Existing model plant lines transformed with known antifungal genes are currently our resources to identify the mechanisms employed by the transgenic plant lines to overcome disease susceptibility upon fungal attack. This information will ultimately lead to the generation of superior antifungal constructs that could be used in genetic transformation protocols.

Poster(s)
Louw, G D, Vivier, M A and Goussard, P G. 2002. The transformation of grapevine cultivars with antifungal genes. Poster presented at the 26th National Congress of the South African Society for Enology and Viticulture. 13-15 November, Somerset West, South Africa.

Presentation(s)
Louw, G D, Vivier, M A and Goussard, P G. 2002. The transformation of grapevine cultivars with antifungal genes. Paper presented at the 26th National Congress of the South African Society for Enology and Viticulture. 13-15 November, Somerset West, South Africa.

Vivier, M A, De Ascensao A, Carstens M, Basson E, Becker J and Pretorius, I S. 2004. Isolation and evaluation of anti-fungal genes for use in grapevine biotechnology. Paper presented at the 7th International Symposium on Grapevine Physiology and Biotechnology, California, United States

Joubert, D A and Vivier, M A. 2004. A polygalacturonase inhibiting protein from Vitis vinifera affects polygalacturonase activity from Botrytis cinerea in vivo, irrespective of specificity. Paper presented at the 7th International Symposium on Grapevine Physiology and Biotechnology. June, California, United States.

Joubert, D A and Vivier, M A. 2004. The activity of endo-polygalacturonase II from Botrytis cinerea is adversely affected in vivo, but not in vitro by the Vitis vinifera polygalacturonase inhibiting protein (VvPGIP1). Paper presented at the 13th International Botrytis Symposium. 25-30 October 2004, Antalya, Turkey.

Vivier, M A, Carstens, M, Basson, E, De Beer, A and Becker, J. 2004. Isolation and evaluation of various antifungal genes for use against Botrytis cinerea in biotechnology approaches. Paper presented at the 13th International Botrytis Symposium. 25-30 October, Antalya, Turkey.

De Beer, A and Vivier, M A. 2004. Isolation and characterisation of a defensin related gene, Vv-AMP1, from grapevine. Paper presented at the 13th Biennial Congress of the South African Society for Microbiology. April 2004, Stellenbosch, South Africa.

De Beer, A and Vivier, M A. 2004. Overexpression of an antimicrobial peptide from Heuchera Sanguinea in tobacco for inhibiting of fungal pathogens. Paper presented at the 13th Biennial Congress of the South African Society for Microbiology. April 2004, Stellenbosch, South Africa.

Joubert, D A and Vivier, M A. 2004. The transcriptional regulation of a polygalacturonase inhibiting protein (PGIP) from grapevine (Vitis vinifera L.). Paper presented at the 7th International Symposium for Grapevine Physiology and Biotechnology. 21-25 June 2004, Davis, California, United States.

Joubert, D A, De Lorenzo, G, Pretorius, I S and Vivier, M A. 2004. Regulation of Vitis vinifera polygalacturonase inhibiting protein (PGIP) encoding genes. Paper presented at the Joint Congress of the South African Microbiology Society and South African Genetics Society. 4-7 April 2004, Stellenbosch, South Africa.

Vivier, M A and Pretorius, I S. 2004. Innovation in the grapevine industry: The use of biotechnology in cultivar improvement. Paper presented at the 4th World Wine Forum, La Rioja, Spain.

Becker, J, Slaughter A, Deny, K and Vivier, M A. 2005. The contribution of the Vitis vinifera polygalacturonase inhibiting protein towards reduced disease susceptibility in transgenic tobacco. Paper presented at the 1st International Grape Genomics Symposium. 12-14 July 2005, St. Louis, Missouri, United States.

Vivier, M A. 2005. Functional analysis of grapevine genes. Paper presented at the 1st International Grape Genomics Symposium. 12-14 July 2005, St. Louis, Missouri, United States.

Joubert, D A, Kemp, G, Bergmann, C, Van Kan, J A L and Vivier, M A. 2005. Characterisation of the interactions between endo-polygalacturonases (PGs) from B. cinerea and a polygalacturonase-inhibiting protein from grapevine (VvPGIP1) with in vitro and in vivo methodologies. Paper presented at the 1st Botrytis Cinerea Genome Workshop. 6-8 October, Kaiserslautern, Germany.

Vivier, M A, Joubert, D A, Becker, J v W, Wentzel, L, Taylor, K and Young, P R. 2005. Functional analysis of grapevine genes. Paper presented at the 1st International Grape Genomics Symposium. 12-14 July 2005, St. Louis, Missouri, United States.

De Beer, A and Vivier, M A. 2006. Isolation and characterisation of a fruit-specific defence from Vitis vinifera. Paper presented at the 3th European Plant Science Organisation Conference. 28 May – 1 June 2006, Visegrad, Hungary.

Thomas, M R, Sergeant, L, Icc, P, Davies, C, Franks, T, Oss, P K, Tar, C, Clingeleffer, P R, Vivier, M A, Young, P R and Torregrosa, L. 2006. Grapevine functional genomics. Paper presented at the 9th International Conference on Grape Genetics and Breeding. 2-6 July 2006, Udine, Italy.

Ben Amar, A, Cobanov, P, Manthey, T, Krezai, G, Bouzid, S, Ghorbel, A, Vivier, M A and Reustle, G M. 2006. Optimised grapevine transformation and regeneration procedures for the use in functional gene analysis and grapevine biotechnology. Paper presented at the 9th International Conference on Grape Genetics and Breeding. 2-6 July 2006, Udine, Italy.

Becker, J v W, Denby, K J and Vivier, M A. 2006. Investigating polygalacturonase inhibiting protein-mediated disease resistance in transgenic tobacco. Paper presented at the 3th European Plant Science Organisation Conference. 28 May – 1st June 2006, Visegrad, Hungary.

Becker, J v W, Joubert, D A and Vivier, M A. 2006. Transcriptome and phytohormone analysis of the resistance phenotype against Botrytis cinerea in transgenic tobacco overexpressing the Vitis vinifera polygalacturonase inhibiting protein. Paper presented at the 2nd Botrytis Genome Workshop. 2006. Versailles, France.

De Beer, A and Vivier, M A. 2005. A small peptide from Vitis vinifera shows strong antifungal activity against the necrotrophic plant pathogen Botrytis cinerea. Paper presented at the 3rd International Congress of the South African Society for Enology and Viticulture. 28-30 July, Cape Town, South Africa.

Vivier, M A. 2005. Grapevine biotechnology: scope and value for viticulture. Paper presented at the 3rd International Congress of the South African Society for Enology and Viticulture. 28-30 July, Cape Town, South Africa.

De Beer, A and Vivier, M A. 2007. A small peptide from Vitis vinifera, shows antifungal activity towards the necrotrophic plant pathogen Botrytis cinerea. Paper presented at the 14th International Botrytis Symposium. 21-26 October 2007, Cape Town, South Africa.

De Beer, A and Vivier, M A. 2007. A ripening induced defensin gene from Vitis vinifera shows antifungal activity against a broad spectrum of fungal pathogens. Paper presented at the 5th International Table Grape Symposium. 14-16 November 2007, Somerset West, South Africa.

Tredoux, M M, De Beer, A, Mostert, L and Vivier, M A. 2007. The evaluation of the antifungal activity of a Vitis vinifera antifungal peptide. Paper presented at the 14th International Botrytis Symposium. 21-26 October 2007, Cape Town, South Africa

Venter, A, Joubert, D A and Vivier, M A. 2007. The evaluation of a range of Vitis polygalacturonase inhibiting proteins (PGIPs) for their antifungal activity against Botrytis cinerea. Paper presented at the 14th International Botrytis Symposium. 21-26 October 2007, Cape Town, South Africa.

Vivier, M A. 2007. Grapevine biotechnology: scope and value for viticulture and table grape product development. Paper presented at the 5th International Table Grape Symposium. 14-16 November 2007, Somerset West, South Africa.

Article
Joubert, D A, Slaughter, A R, Kemp, G, Becker, J v W, Krooshof, G H, Bergmann, C, Benen, J, Pretorius, I S, Vivier, M A. 2006. The grapevine polygalacturonase-inhibiting protein reduces Botrytis cinerea susceptibility in transgenic tobacco and differentially inhibits fungal polygalacturonases, Transgenic Research, v. 15 (6) (p. 687-702)

Joubert, D A, Kars, I, Bergmann, C, Kemp, G, Vivier, M A, Van Kan, J A L. 2007. A polygalacturonase-inhibiting protein from Vitis vinifera differentially inhibits a Botrytis cinerea endoploygalacturonase in vitro and in vivo, Molecular Plant Microbe Interactions, v. 20 (4) (p. 392-402)

Dissertation
Joubert, D A. 2004. Regulation of the Vitis vinifera PGIP1 gene encoding a polygalacturonase-inhibiting protein. PhD. University of Stellenbosch, Stellenbosch, South Africa.

Becker, J v W. 2007. Evaluation of the role of PGIP in plant defense responses. PhD. University of Stellenbosch, Stellenbosch, South Africa.

Thesis
Wentzel, L. Specificity analysis of the interaction between isozymes of the Vitis vinifera polygalacturose inhibiting protein (PGIP) and endopolygalacturonases (PGs) from Botrytis cinerea. MSc. University of Stellenbosch, Stellenbosch.

Louw, G D. 2005. Specificity analysis of the interaction between isozymes of Vitis vinifera polygalacturonase inhibiting protein (PGIP) and endopolygalacturonases (PGs) from Botrytis cinerea. MSc. University of Stellenbosch, Stellenbosch.

Mbewana, S. 2006. Analysis of lignin levels and a possible fungal resistance phenotype in transgenic tobacco plants overexpressing a native CAD gene. MSc. University of Stellenbosch, Stellenbosch.

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