Funding Support for Transformation and Regeneration Facility for Grapevine: 2008 - 2011


Project Number
IWBT-P 08-14

Project title
Funding support for transformation and regeneration facility for grapevine: 2008 – 2011

Project leader
Vivier, M A

Institution
University of Stellenbosch. Faculty of AgriSciences. Institute for Wine Biotechnology

Project description
The establishment of techniques, resources and in particular the human skills base for a grapevine transformation and regeneration programme was a tremendous challenge and a long term effort.

The necessary human skills, technological resources, infrastructure, methodologies and techniques to perform genetic transformation of grapevine have been established and optimised at the IWBT and are an indispensable base for scientific investigation of the grapevine.

The following has been achieved:
1. Tissue culture techniques, transformation, selection and regeneration technologies were established for commercially important wine and table grape cultivars as well as rootstock varieties;
2. Optimisations to several methods were introduced, including: cryopreservation for long term maintenance and storage of cell lines, the optimisation of suspension cell cultures for use in transformation protocols and the improvement of transformation and regeneration protocols to routinely yield transgenic plants three to six months post transformation.

Presentation(s)
Vasanth, K, Lerm, T and Vivier, M A. 2008. Cryopreservation and Agrobacterium mediated transformation of suspension cultures of grapevine (Vitis vinifera). Paper presented at the International Conference of Biotechnology. 6-8 February, India

Vasanth, K and Vivier, M. 2008. Grapevine tissue culture and transformation. Paper presented at the 4th International Symposium on Acclimatization and Establishment of Micro-propagated Plants. 8-12 December, Bangalore, India.

Du Preez, J, Stephan, D, Blignaut, M, Stander, C, Vivier, M A, Goszczynski, D and Burger, J T. 2008. The characterisation of virus-based vectors for functional genomic studies in grapevine. Paper presented at the 8th International Symposium on Grapevine Physiology and Biotechnology. 23-28 November, Adelaide, Australia,

Freeborough, M-J, Malan, S, Stander, C, Vivier, M A and Burger, J T. 2008. Molecular analysis of transgenic grapevine plants containing a virus resistance construct. Paper presented at the 31st National Congress of the South African Society for Enology and Viticulture. 11-14 November, Somerset West, South Africa.

Stander, C and Vivier, M A. 2008. Grape berry suspension cultures as potential tools to model ripening related processes. Presentation to the Cape Biotechnology Forum. 30 November – 2 December, Somerset West, South Africa.

Stander, C, Vasanth, K, Korkie, M and Vivier, M A. 2009. In vitro culture systems to study and genetically improve grapevine (Vitis vinifera) cultivars. Paper presented at the 16th South African Society for Microbiology Conference and BIO-2-BIZ Conference. 20-23 September, Durban, South Africa.

Stephan D, Du Preez, J, Stander, C, Vivier, M A, Muruganantham, M, Mawassi, M and Burger, J T. 2009. Vacuum-agroinfiltration of different Vitis vinifera cultivars and application of VIGS in the cv. Sultana. Paper presented at the 16th Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine., 31 August – 4 September, Dijon, France.

Sharathachandra, R G, Stander, C, Ndima, B and Vivier, M A. 2010. Optimising a proteomics approach to evaluate grapevine berry suspension cultures. Paper presented at the 15th Australian Proteomics Conference, Lome, Australia

Sharathachandra, R G, Stander, C, Ndima, B and Vivier, M A. 2010. Proteomic analysis of secreted proteins during grape berry. Paper presented at the Human Proteome Organisation’s World Congress, Sidney, Australia

Vivier, M A and Young, P R. 2011. Genetically modified organisms grapevines: excellent study models and promising (future) products. Paper presented at the 2nd Genetically Modified Organisms in Horticulture Symposium. 11-15 September 2011, White River, South Africa.

Article
Ramaschandra, S G, Stander, C, Jacobson, D, Ndimba, B K, Vivier, M A. 2011. Proteomic analysis of grape berry cell cultures reveals that developmentally regulated ripening related processes can be studied using cultured cells, Plos One, v. 6 (2) (p. e14708)

Vasanth, K, Vivier, M A. 2011. Improved cryopreservation procedure for long term storage of synchronised culture of grapevine, Biologia Plantarum, v. 55 (2) (p. 365-369)

FinalReport.pdf

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