Development of PCR-based Test for Identification of Asymptomatic Black Dead Arm and Eutypa Dieback Infections from Vineyard and or Nursery Material


Project Number
240031

Project Title
Development of PCR-based test for identification of asymptomatic Black dead arm and Eutypa dieback infections from vineyard and or nursery material

Project Leader
Safodien, S

Team Members
Fourie, P
Crous, P W

Project Description
Fungal diseases are largely responsible for grapevine decline causing extensive losses in production yields. This presents a major problem for the vine and wine industry. Eutypa dieback (Eutypa lata / Eutypa armeniacae) and black dead arm (Botryosphaeria spp.) are responsible for infections of asymptomatic nursery material. The grapevine trunk diseases caused by Eutypa lata and the Botryosphaeria spp. have become an important area of study because of their devastating impact on vineyards worldwide. These canker diseases may limit the long term sustainability of wine and grape production by limiting the productivity of new vines and can be costly because of the expense of vine destruction, replanting, and importantly, the magnitude of yield losses that can occur.

Because the diseases appear to be latent, it becomes vital for farmers to know that cuttings are healthy before planting them in vineyards. Subsequently it is important to develop an identification method of these diseases even on young nursery material that are asymptomatic. The development of a molecular technique for screening nursery material and isolates would be important not only in fungicide screening but in screening for resistant rootstocks too (it is known that certain cultivars show varying levels of susceptibility to the diseases). With a view to developing a screening technique that would provide fast, accurate and reliable means of detecting plant pathogens, reverse dot blot technology appears promising.

Presentation(s)
Arendse, M, Safodien, S, Snyders, C, Smit, L and Smit, W A. 2002. Identification of Phytophthora cinnamomi by reverse dot blot hybridisation. Paper presented at the 40th Congress of the South African Society for Plant Pathology. 20-23 January, Brits, South Africa.

Safodien, S, Halleen, F, Smit, L, Crous, P W and Smit, W A. 2002. The status of Eutypa on grapevine in South Africa. Paper presented at the 26th National Congress of the South African Society for Enology and Viticulture. 13-15 November, Somerset West, South Africa.

Safodien, S, Arendse, M, Langenhoven, W E, Mkhize, T M, Snyders, C S, Smit, L and Smit, W A. 2002. Development of a one stop molecular diagnostic tool for detection and identification of plant pathology. Paper presented at the 2nd Cape Biotechnology Forum, 30 November – 2 December, Somerset West, South Africa.

Safodien, S, Arendse, M, Halleen, F, Crous, P W, Botha, A and Smit, W A. 2003. Development of a PCR-based assay for the identification of Eutypa lata from grapevines. Paper presented at the 27th National Congress of the South African Society for Enology and Viticulture. 5-7 November, Somerset West, South Africa.

Safodien, S, Smit, L, Halleen, F, Groenewald, J Z, Crous, P W, Botha, A and Smit, W A. 2003. The characterisation of Eutypa isolates associated with Eutypa dieback of grapevines in South Africa. Paper presented at the 41st Congress of the South African Society for Plant Pathology. 19-22 January, Bloemfontein, South Africa.

Safodien, S, Smit, L, Halleen, F, Groenewald, J Z, Crous, P W, Botha, A and Smit, W A. 2003. The characterisation of Eutypa isolates associated with Eutypa dieback of grapevines in South Africa. Paper presented at the 3rd International Workshop on Grapevine Trunk Diseases. 1-2 February, Lincoln University, New Zealand.

Safodien, S, Arendse, M, Halleen, F, Crous, P W , Botha, A and Smit, W A. 2004. Development of a PCR-based assay for the identification of Eutypa lata from grapevines. Paper presented at the 42nd Congress of the South African Society for Plant Pathology. 18-21 January, Winterton, Kwazulu-Natal.

Langenhoven, W E, Arendse, M S, Mkhize, T M, Smit, L, Smit, W A and Knoetze, R. 2004. Reverse dot blot hybridisation as a diagnostic tool for detection and identification of grapevine pathogens. Paper presented at the 42nd Congress of the Southern African Society for Plant Pathology. 18-21 January, Winterton, South Africa.

Safodien, S, Halleen, F, Crous, P W, Botha, A and Smit, W A. 2005. Molecular characterisation and identification of Eutypa spp. from grapevines in South Africa. Paper presented at the 4th International Workshop on Grapevine Trunk Diseases. 20-21 January, Stellenbosch, South Africa.

Safodien, S, Halleen, F, Crous, P W, Botha, A and Smit, W A. 2005. Detection of Eutypa lata by reverse dot blot hybridisation. Paper presented at the 4th International Workshop on Grapevine Trunk Diseases. 20-21 January 2005, Stellenbosch, South Africa.

Safodien, S, Halleen, F, Crous, P W, Botha, A and Smit, W A. 2005. Detection of Eutypa lata by reverse dot blot hybridisation. Paper presented at the 43rd Congress of the Southern African Society for Plant Pathology, African Mycology Association and Medical Mycology in Africa. 23-26 January, Hartenbos, South Africa.

FinalReport.pdf

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