The Effect of Hot Water Treatment (50°C for 45 min) on Fungal Pathogens in Propagation Material and Nursery Vines


Project Number
P04000105

Project title
The effect of hot water treatment (50°C for 45 min) on fungal pathogens in propagation material and nursery vines

Project leader
Halleen, F

Team members
Mostert, L
Lesuthu, P
Vermeulen, C
Marais, J
Marais, D

Project description
The effect of Hot Water Treatment (50°C for 45 min) on the occurrence of fungal pathogens associated with grapevine propagation material and nursery vines has not been determined under South African conditions. Previous information was based on the recommendation of 50°C for 30 min as employed by South African nurseries. Although this treatment was effective in reducing Pythium, Phytophthora and most trunk pathogens, we know from previous research that this treatment did not eradicate all infections. Furthermore, previous research has also shown that these infections could increase to pre-treatment levels within 3 years after establishing a new vineyard. International literature is inadequate because studies were done with other rootstock varieties (or only with a small selection of rootstocks used in South Africa), or the studies tested only one or two pathogen species whilst we know that several fungal groups have the ability to infect South African vines.

The aim of the study will, therefore, be to determine the effect of Hot Water Treatment (50°C for 45 min) on the occurrence of fungal pathogens associated with grapevine propagation material and nursery vines in order to ensure complete eradication by these harmful pathogens and to prevent re-colonisation.

The Objectives
To determine the effect of HWT (50°C for 45 min) on the survival of fungal pathogens (in vitro).
To determine the effect of HWT on the eradication of fungal pathogens inside propagation material.
To determine the effect of HWT on the eradication of fungal pathogens inside dormant grafted vines.

– Record end –

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